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More information:Visualizing cytoskeletal networks in Drosophila mitotic neuroblasts using GFP-tagged spectraplakin proteins. Drosophila neuroblasts divide at their apical ends and generate a neuron at the basal end of the dividing cell. During mitosis, neuroblasts reorganize their actin cytoskeleton to form the mitotic spindle and an apical membrane domain at the posterior cell end. Moreover, the neuroblast cell cortex undergoes a rearrangement to form a basal cell surface domain at the anterior cortex. In order to understand how neuroblasts orient their growth axes, we have used genetic and imaging tools to investigate the organization of the neuroblasts' cytoskeletal networks. We have used GFP-tagged versions of fasciclin II and IIB (FasII and FasIIB), spectraplakin proteins, which form spectrosome-like organelles in these cells. We show that the spectrosome-like organelles are distributed throughout the neuroblasts during interphase and at the basal cortex of mitotic cells. During mitosis, FasII and FasIIB were concentrated at the basal cortex, forming a cortical ribbon structure. Furthermore, FasII was associated with the mitotic spindle and F-actin. In contrast, FasIIB concentrated at the basal cortex formed filaments associated with the basal cortex during interphase. We have also used a genetic strategy to construct flies that express FasIIB-GFP in a cell-autonomous manner and to show that this protein colocalizes with the cortical spectrosome-like organelles in larval brain neuroblasts.Is Shingles a Risk for Pregnant Women? Shingles can cause a painful skin rash in anyone who has a suppressed immune system, such as a person with HIV or cancer, or has recently received a transplant. But does it also affect pregnant women? Shingles is a viral infection that, although most people are infected by 20 years of age, it can return for many years. The virus travels to the basal nerve cells that send messages to and from the skin. 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